Magnesium chloride for PCR ROX additive & gel loading buffer

PCR-grade magnesium chloride is used to optimise the Mg2+ ion concentration in PCR and qPCR reactions. The ROX Additive solution for qPCR is most commonly used as a passive reference dye that is used to reduce well-to-well variations in fluorescence during real time PCR data acquisition. Our 6x Sample Loading Buffer is a gel loading solution containing two tracking dyes that allows tracking of DNA migration in gel electrophoresis workflows.

Features

• PCR Grade Purity: Ensures high specificity and yield in PCR applications.
• Ready-to-Use: Pre-prepared solutions for immediate integration into your workflows.
• Flexible Concentrations: suitable for various PCR and qPCR setups.
• Ideal for custom assay development
• Replace kit components affordably

Applications

• Standard PCR and qPCR assays.
• Multiplex PCR reactions.
• Gel electrophoresis
• Restriction fragment analysis

Magnesium Chloride Solution (PCR Grade): 

Optimized for PCR reactions, our Magnesium Chloride Solution ensures consistent and efficient amplification in standard and multiplex PCR applications. It is supplied in a ready-to-use format and can enhance enzyme activity and amplification specificity as a required co-factor of DNA-manipulating enzymes, including DNA polymerases. This 50 mM solution is intended for optimisation of the Mg2+ ion concentration in PCR and qPCR reactions.

PCR Biosystems polymerase kits and mixes are supplied with an optimised magnesium chloride concentration and generally do not require any further addition. However, some assays may require further customisation. The ideal magnesium concentration in individual reactions depends on the template, buffer, and dNTPs. Further addition should be carefully titrated and reaction performance compared to the unmodified reaction.

ROX Additive Solution (qPCR Passive Reference Dye):

Enables precise real-time PCR data normalization. Our ROX additive is a stabilised conjugate of 5-carboxy-X-rhodamine ester used as a passive reference dye to normalise fluorescent signal and correct for well-to-well variation in real time qPCR. The dye is supplied at a 50 μM concentration that can be used as a 100x stock solution for instuments requiring high ROX concentration (500 nM) and as a 1000x stock for instruments requiring a low ROX concentration (50 nM).

PCR Biosystems supplies most qPCR products in premixed, separate tube and no-ROX formulations for maximum ease of use. This product is indended for use when additional ROX is required and for the development of bespoke assays.

Gel Loading Buffer (6x Sample Loading Buffer):

Facilitates accurate and efficient DNA/RNA sample visualization with our 6x Gel Loading Buffer, designed for electrophoresis workflows. This buffer contains xylene cyanol, bormophenol blue and glycerol for sharp band visualisation and retains nucleic acids in wells when setting up electrophoresis experiments. It is suitable for both agarose and polyacrylamide gels and can be used with all PCR Biosystems endpoint reagents and standard PCR products from other suppliers that do not already contain a gel loading dye (e.g., PCRBIO Red Mixes). The two tracking dyes migrate at approximately 150 bp and 800 bp on a 2% TAE agarose gel or at approximately 500 bp and 4,000 bp on a 1% TAE agarose gel.